Description
CoproELISA C. difficile ToxA/B is an enzyme immunoassay for the detection of toxin A and toxin B in human feces. Break-apart microwells are coated with C. difficile toxin-specific polyclonal antibodies. A set of horseradish peroxidase (HRP) conjugated polyclonal anti-toxin A and anti-toxin B antibodies are added to the antibody-coated microwells. Fecal samples are diluted in sample diluent and added to the microwells. In this step C. difficile toxins are specifically marked by the HRP conjugated antibodies and immobilized by the coated antibodies. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the level of C. difficile toxins in the sample